• Prevents cell loss,

Figure 3: Transfection procedure with FuGENE^® HD Transfection Reagent.  Please note that the procedure does not require a media change after step 2.

• Generates high levels of protein expression in many adherent and suspension-adapted eukaryotic cell lines , including HEK-293 （Figure 4a）, Hep G2, CHO-S, CHO-K, COS-7, COS-1, and insect cell lines such as High Five （Figure 4b） and Sf9. 

 

Figure 4a: High transfection efficiency and protein expression in suspension-adapted mammalian cells.
Panel a: HEK-293 EBNA suspension-adapted cells were transfected with plasmid DNA expressing GFP. The transfection reagent / DNA ratios used were 7:2, 6:2, 5:2, 4:2, and 3:2.
Panel b: The percentage of cells transfected was determined 28 hours post-transfection, and the quantity of GFP protein was estimated on a Coomassie-stained gel at 72 hours post-transfection.
* estimated yield
 

Figure 4b: High transfection efficiency and protein expression in High Five insect cells.
Panel a: Cells were transfected with a vector containing the LacZ gene. Transfection  efficiency was demonstrated 24 hours post-transfection by histochemical staining for β-galactosidase activity （β-Gal Staining Set）
Panel b: Cells were transfected with vectors expressing human kinases Akt1 and MKK6. Protein expression in cell lysates was measured by western blot at 72 hours post-transfection.