TRIzol® Reagent

（Ambion®）

描述

TRIzol® 試劑是一種*的即用型試劑，可用于提取高質(zhì)量的總RNA或者從各種生物樣本中同時(shí)提取RNA、DNA和蛋白質(zhì)。這種酚和異硫氰酸胍單相溶液，可用于提取人、動(dòng)物、植物、酵母或細(xì)菌來源的細(xì)胞和組織樣本中的純RNA、DNA和蛋白質(zhì)，只需一個(gè)小時(shí)即可完成。

• 從一份樣品同時(shí)提取RNA、DNA和蛋白質(zhì)

• 即便是難以處理的樣本類型，亦具有出色的裂解能力

• 優(yōu)化的配方和實(shí)驗(yàn)方案，適用于組織、細(xì)胞、血清、病毒和細(xì)菌等多種樣品類型

從多種樣本體積和來源獲得可靠的純化RNA

TRIzol® 試劑用于處理人、動(dòng)物、植物或細(xì)菌來源的少量組織（50-100 mg）和細(xì)胞（5 Ã— 10⁶），以及大量組織（≥1 g）和細(xì)胞（>10⁷），均可獲得的性能，且附帶樣本純化實(shí)驗(yàn)方案。在TRIzol® 試劑進(jìn)行樣本勻漿化的過程中，它可以破壞細(xì)胞，溶解細(xì)胞組分，同時(shí)高效的抑制RNA酶的活性，從而維持RNA的完整性。TRIzol® 試劑方法十分簡單，可同時(shí)處理大量樣本。整個(gè)過程可在一小時(shí)內(nèi)完成。采用TRIzol® 試劑提取的總RNA不會(huì)被蛋白質(zhì)和DNA污染。

適用于多個(gè)分子靶點(diǎn)的提取

TRIzol® 試劑使您可以順序沉淀單一樣本中的RNA、DNA和蛋白質(zhì)。采用TRIzol® 試劑進(jìn)行樣本勻漿化后，加入氯仿，勻漿物可分成透明的上層水相層（含有RNA）、相界面和紅色的下層有機(jī)層（含有DNA和蛋白質(zhì)）。然后用異丙醇從水相層中沉淀出RNA。用從相界面/有機(jī)層中沉淀出DNA。利用異丙醇沉淀從酚-上清液中沉淀出蛋白質(zhì)。洗滌沉淀的RNA、DNA或蛋白質(zhì)，去除雜質(zhì)，重懸浮后供下游應(yīng)用。

僅供研究使用。不得用于動(dòng)物或人類的診斷或治療。

詳細(xì)說明

常用規(guī)格

Sample Type （General）:	Viral Samples, Plant Samples, Tissue, Blood, Bacteria, Yeast, Cells
Final Product:	Transcriptome RNA, Total RNA, micro RNA
Downstream Application:	Northern Blotting, Nuclease Protection Assays, Real-Time PCR, cDNA Library Construction, Cloning, RT-PCR （Endpoint）
High Throughput Compatibility:	Not High Throughput-Compatible, Manual Protocols
Isolation Technology:	Organic Extraction
Scale:	Scaleable
System:	TRIzol
Quantity:	1
Product Size:	100 ml
Purification Time:	1 hr
Number of Reactions:	100 Preps
Starting Material （Amount）:	Up to 1 g tissue, Up to 1 x 10^7 cells
Shipping Condition:	Room Temperature
Regulatory Statement:	For Research Use Only. Not for use in diagnostic procedures.

額外信息

引文和參考文獻(xiàn) （258）

Elevated mitochondrial reactive oxygen species generation affects the immune response via hypoxia-inducible factor-1alpha in long-lived Mclk1+/- mouse mutants.

作者: Wang D, Malo D, Hekimi S,
雜志: J Immunol （2010） 184:582-590
產(chǎn)品用途: Measurement of H2O2 secretion and mitochondrial superoxide generation in RAW264.7 cells and mouse peritoneal macrophages.
ID:: PN67730

Programmable in situ amplification for multiplexed imaging of mRNA expression.

作者: Choi HM, Chang JY, Trinh le A, Padilla JE, Fraser SE, Pierce NA,
雜志: Nat Biotechnol （2010） 28:1208-1212
產(chǎn)品用途: Amplified in situ detection of mRNAs via hybridization-initiated self-assembly of fluorescent RNA hairpins.
ID:: PN68638

In vivo imaging of zebrafish digestive organ function using multiple quenched fluorescent reporters.

作者: Hama K, Provost E, Baranowski TC, Rubinstein AL, Anderson JL, Leach SD, Farber SA,
雜志: Am J Physiol Gastrointest Liver Physiol （2009） 296:G445-G453
產(chǎn)品用途: Simultaneous visualization of intestinal lipase and protease activity in live zebrafish.
ID:: PN67267

Human benign prostatic hyperplasia stromal cells as inducers and targets of chronic immuno-mediated inflammation.

作者: Penna G, Fibbi B, Amuchastegui S, Cossetti C, Aquilano F, Laverny G, Gacci M, Crescioli C, Maggi M, Adorini L,
雜志: J Immunol （2009） 182:4056-4064
產(chǎn)品用途: （1） RNA extraction for real-time quantitative RT-PCR. （2） Confocal immunofluorescence analysis of of primary human benign prostatic hyperplasia （BPH） cells. （3） Analysis of CD4+ T cell proliferation in co-culture with BPH cells.
ID:: PN67481

Dynamin 2 mutations associated with human diseases impair clathrin-mediated receptor endocytosis.

作者: Bitoun M, Durieux AC, Prudhon B, Bevilacqua JA, Herledan A, Sakanyan V, Urtizberea A, Cartier L, Romero NB, Guicheney P,
雜志: Hum Mutat （2009） 30:1419-1427
產(chǎn)品用途: Analysis of the impact of Dynamin 2 GTPase mutations on LDL and transferrin receptor endocytosis.
ID:: PN67795

更多..

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按細(xì)胞系排列的引文

細(xì)胞系名稱	有機(jī)體類型	組織
MCF-7	Human	Breast/Mammary
P19 cells	Mouse	Embryo
Smooth muscle cells （vascular）	Rat	Muscle
U-937	Human	Macrophage
HAECs	Human	Aorta
THP-1	Human	Blood
TIG	Human	Fibroblast

主營：生化試劑、抗體、重組蛋白、細(xì)胞生物、分子生物、蛋白研究、微生物、Elisa試劑盒、耗材、標(biāo)準(zhǔn)品、標(biāo)準(zhǔn)溶液……

abcam、ABI、Axygen、corning、信號轉(zhuǎn)導(dǎo)抗體、Gibco、Hyclone、invitrogen、merck、Millipore、Qiagen、R&D、Roche、santa、sigma、Thermo等進(jìn)口品牌……

以下為本公司常備現(xiàn)貨產(chǎn)品

*部分 invitrogen 原裝產(chǎn)品

品牌	貨號	名稱	規(guī)格
invitrogen	13778-075	Lipofectamine RNAiMAX	0.75ml
invitrogen	13778-150	Lipofectamine RNAiMAX	1.5ml
invitrogen	11668-027	Lipofectamine 2000 Reagent	0.75ml
invitrogen	11668-019	Lipofectamine 2000 Reagent	1.5ml
invitrogen	15596-026	TRlzol ®Reagent	100ml
invitrogen	15596-018	TRlzol ®Reagent	200ml
invitrogen	10296-010	Trizol ® LS Reagent	100ml
invitrogen	10296-028	Trizol ® LS Reagent	200ml
invitrogen	17504-044	B-27（黃顏色）	10ml
invitrogen	12587-010	B-27（黃顏色）不含維生素A	10ml
invitrogen	17502-048	N-2 （粉紅顏色）	5ml
invitrogen	18064-014	SuperScript® II Reverse Transcriptase	10,000U
invitrogen	18080-044	SuperScript® III Reverse Transcriptase	10,000U

第二部分 HyClone胎牛血清

品牌	貨號	名稱	規(guī)格
HyClone	SH30370.03	Australian Standard Fetal Bovine Serum	500ml
HyClone	SH30406.02	FOETAL BOVINE SERUM	500ml
HyClone	SH30401.01	New Zealand Calf Serum	500ml
HyClone	SH30066.02	FetalClone® II	100ml
HyClone	SH30088.03	STANDARD FETAL BOVINE SERUM	500ml
HyClone	SH30118.02	Newborn Bovine Calf Serum, U.S. Origin	100ml
HyClone	SH30070.02	FETAL BOVINE SERUM	100ml
HyClone	SH30087.02	COSMIC CALF® SERUM	100ml
HyClone	SH30070.03E	FETALBOVINESERUM CHARACTERIZSED	500ml
100ml	SH30070.03E	FETALBOVINESERUM CHARACTERIZSED
HyClone	SH30070.03	FETAL BOVINE SERUM	500ml
HyClone	SH30080.03	FetalClone® I	500ml
HyClone	SH30084.03	FOETAL BOVINE SERUM: CERTIFIED AUSTRALIAN	500ml
HyClone	SH30396.03	FETAL BOVINE SERUM CHARACTERIZED	500ml
HyClone	SV30087.02	南美源胎牛血清	500ml
HyClone	sv30087.01	南美源胎牛血清	100ml
HyClone	SH30071.03	FETAL BOVINE SERUM	500ml
HyClone	SH30071.02	FETAL BOVINE SERUM	100ml
HyClone	SH30080.02	FetalClone® I	100ml
HyClone	SH30070.02E	FETAL BOVINE SERUM CHARACTERIZSED	100ml
HyClone	SH30079.02	FETAL BOVINE SERUM	100ml
HyClone	SH30068.02	CHARCOAL/DEXTRAN TREATED FBS，活性炭滅活血清	100ml
HyClone	SH30068.03	CHARCOAL/DEXTRAN TREATED FBS，活性炭滅活血清	500ml
HyClone	SH30066.03	FetalClone® II	500ml
HyClone	SH30109.03	HyClone FetalClone® III	500ml
HyClone	SH30088.03	STANDARD FETAL BOVINE SERUM	500ml
HyClone	SH30088.02	STANDARD FETAL BOVINE SERUM	100ml
HyClone	SH30079.03	FETAL BOVINE SERUM	500ml

第二部分 Gibco胎牛血清

品牌	貨號	名稱	規(guī)格
Gibco	12662-029	MSC QUALIFIED FBS 500ML	500ML
Gibco	10438-026	FBS, HI 500ML	500ML
Gibco	10439-024	FBS, ES CELL QUAL. USDA APPD. 500ML	500ML
Gibco	10099-141	FBS, AUSTRALIAN ORIGIN 500ML	500ML
Gibco	26400-044	FBS, DIALYZED, USA ORIGIN 500ML	500ML
Gibco	26140-079	FBS QUALIFIED, USA ORIGIN 500ML	500ML
Gibco	16141-079	FBS, ES CELL QUAL, USA ORIGIN 500ML	500ML
Gibco	16140-071	FBS, HI 500ML	500ML
Gibco	16000-044	FBS, CERT, USA ORIGIN 500ML	500ML
Gibco	C2027050	FBS, URUGUAYAN ORIGIN	500ML
Gibco	12657-029	巴西胎牛	500ml

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上海博耀生物科技有限公司

上海博耀生物科技有限公司

TRIzol Reagent說明書，Ambion（15596-026）返回列表頁

TRIzol® Reagent

描述

詳細(xì)說明

常用規(guī)格

額外信息

引文和參考文獻(xiàn) （258）

Elevated mitochondrial reactive oxygen species generation affects the immune response via hypoxia-inducible factor-1alpha in long-lived Mclk1+/- mouse mutants.

Programmable in situ amplification for multiplexed imaging of mRNA expression.

In vivo imaging of zebrafish digestive organ function using multiple quenched fluorescent reporters.

Human benign prostatic hyperplasia stromal cells as inducers and targets of chronic immuno-mediated inflammation.

Dynamin 2 mutations associated with human diseases impair clathrin-mediated receptor endocytosis.

按細(xì)胞系排列的引文

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精品乱码一区内射人妻无码-亚洲中文AⅤ中文字幕在线-免费不卡国产福利在线观看-国产综合无码一区二区色蜜蜜

上海博耀生物科技有限公司

上海博耀生物科技有限公司

TRIzol Reagent說明書，Ambion（15596-026） 返回列表頁

TRIzol® Reagent

描述

詳細(xì)說明

常用規(guī)格

額外信息

引文和參考文獻(xiàn) （258）

Elevated mitochondrial reactive oxygen species generation affects the immune response via hypoxia-inducible factor-1alpha in long-lived Mclk1+/- mouse mutants.

Programmable in situ amplification for multiplexed imaging of mRNA expression.

In vivo imaging of zebrafish digestive organ function using multiple quenched fluorescent reporters.

Human benign prostatic hyperplasia stromal cells as inducers and targets of chronic immuno-mediated inflammation.

Dynamin 2 mutations associated with human diseases impair clathrin-mediated receptor endocytosis.

按細(xì)胞系排列的引文

智能制造網(wǎng)

會(huì)員登錄

收藏該商鋪

提示

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TRIzol Reagent說明書，Ambion（15596-026）返回列表頁