The EasyClone Normalized cDNA Library Construction Kit is designed to synthesize full-length-enriched
double stranded （ds） cDNA from total or polyA+ RNA, followed by normalization of amplified cDNA and subcloning into an appropriate library vector. The kit is based on a novel technology utilizing the specific features of MMLV-based reverse transcriptase to create full length enriched double stranded cDNA from total or polyA+ RNA samples. cDNAs are amplified by representational PCR and cloned directionally into library vectors for use with yeast two-hybrid systems such as Dualsystems' DUALmembrane, DUALhybrid, and DUALhunter protein interaction screening kits.

 

 

No extensive know-how in library construction is needed - the kit supplies all reagents needed for library construction and the detailed manual will guide you through all steps of construction.

 

Normalization is the process of removing abundant RNAs from the starting material. Normalized cDNA libraries contain approximay equal amounts of all RNAs and therefore increase the occurrence of rare proteins. This significantly increases the quality of yeast two-hybrid screens.

 

Figure 1: qPCR of normalized and not normalized human spleen cDNA. For Actin, a decrease

of 75.8% was reached. For hGAPDH, the decrease was 60.6%. The amount of these two high

abundant cDNAs was lowered, increasing the quality of the cDNA library.

 

 

 

 

如果您對本品感興趣請與我公司
北京東方安諾生化科技有限公司
：蔡
：
：http://www.annovatech。。ｃｏｍ/
：  573795257